Efficient IDUA Gene Mutation Detection with Combined Use of dHPLC and Dried Blood Samples

Efficient IDUA Gene Mutation Detection with Combined Use of dHPLC and Dried Blood Samples

Objectives. Development of a simple mutation directed method in order to allow lowering the cost of mutation testing using an easily obtainable biological material. Assessment of the feasibility of such method was tested using a GC-rich amplicon. Design and Methods. A method of denaturing high-performance liquid chromatography (dHPLC) was improved and implemented as a technique for the detectio...

Research Proposal: Mycotoxin Detection in Dried Blood Description Development and Validation of Methods for Detection of Mycotoxins Exposure in Dried Spotted Blood Samples Project Investigator

Study and detection of ERG11 gene mutation in resistant - drug Candida albicans and relation with Iranian women infertility

Aim and Background: Candida  Albicans is the most important cause of  vulvovaginal candidiasis and resistance to azoles can occur via various mechanisms including, change in the ERG11 gene. This study aim was to identification  of  ERG11 gene mutations in drug  resistant Candida albicans isolated from patients with Candida vaginitis and its association with infertility in Iranian women.   Mat...

detection of volatile compounds of medicinal plants with some nano-sorbents using modified or new methodologies and investigation of antioxidant activity of their methanolic extracts

in this work, a novel and fast method for direct analysis of volatile compounds (davc) of medicinal plants has been developed by holding a filament from different parts of a plant in the gc injection port. the extraction and analysis of volatile components of a small amount of plant were carried out in one-step without any sample preparation. after optimization of temperature, extraction time a...

The Use of Dried Blood Spot Samples in Screening Drugs of Abuse

The present article will provide an overview of use of dried blood sampling method for analysis of drug of abuse. Relatively short half life and instability in blood, calls for alternative sampling method for determination of drugs of abuse. Dried blood spot (DBS) method has many advantages over the conventional sampling methods. The available method for DBS sample collection, storage and trans...